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Polymer Factory’s new IM-MS-designated tuning and calibration kit is based on hydrophilic dendritic macromolecules that cover the m/z range between 605 and 2,390 and the CCS range of 220 - 975 Å2.

 

 

This mixture of 9 hydrophilic, dendritic components is delivered as a dried down, neat solid. Recommended ESI concentration is 10 µg/mL in polar solvents, such as ethanol, methanol, THF (or their aqueous mixtures), water, aqueous acetonitrile.

 

The 9 components will generate ions of charge states 1-4, generating a m/z range between 605 and 2,390 and a CCS range between 220 and 975 Å2.

Component IDChemical Formulazm/z [M+zNa]z+DTCCSN2 [Å2]
PFS-1042C25H44O16z = 1623.2527220.65
PFS-3012C37H62O22z = 1881.363287.23
PFS-2032C50H84O30z = 11187.4945317.41
PFS-2032C50H84O30z = 2605.2421351.19
PFS-2042C65H108O40z = 11551.6315366.74
PFS-2042C65H108O40z = 2787.3106388.89
PFS-2052C80H132O50z = 11915.7684411.72
PFS-2052C80H132O50z = 2969.3791438.51
PFS-2062C100H166O61z = 21194.4836494.35
PFS-2062C100H166O61z = 3803.986519.29
PFS-3042C145H236O88z = 21715.6888600.23
PFS-3042C145H236O88z = 31151.4556625.48
PFS-3052C180H292O110z = 22129.852673.77
PFS-3052C180H292O110z = 31427.5644703.57
PFS-4042C305H492O184z = 32390.2972(4)950.54
PFS-4042C305H492O184z = 41798.4702(4)975.23

 

Observe that intensities, charge states, H+/Na+ and their distribution can vary depending on the hardware, spraying conditions and tuning.

 

What makes SpheriCal® IM-MS unique and superior to other tuning and calibration mixes?

In contrast to other calibration solutions, we offer our calibrants as solid, neat compound. The material is fully soluble in hydrophilic solvents commonly used in ESI. This includes water, alcohols, aqueous mixtures of alcohols, aqueous ACN.

Our compounds are “non-sticky” in the ion source and will not tail into your data. At the same time, the compatibility with a wide range of spraying solutions and conditions enables internal calibration.

Further, SpheriCal® does not contain any fluorinated components.

With a shelf life of years, we expect the 10 µg portions to enable about 100 calibrations.

SpheriCal® offers reliable and accurate calibration. Thanks to the extended calibration space in the CCS vs m/z plane, there is no need to extrapolate from singly charged molecules with limited molecular masses.

 

In positive mode, our compounds do not need addition of acetic or formic acid and ionize well with traces of Na to yield [M+Na]+. Addition of acid will also yield [M+H]+ in positive mode.

In negative mode, addition of acid is required and yields [M+acid-H]- anions. [M-H]- might also be observed.

This gives full control to the end user to choose concentration, solvent, addition of acid etc.

 

 

The feasabilty on several IM-MS platforms has been studied and published in the Journal of the American Society for Mass Spectrometry

 

 

Related Publications

E.R. Sekera, J. Sommertune et. al.: Utilization of bis-MPA Dendrimers for the Calibration of Ion Mobility Collision Cross Section Calculations, J. Am. Soc. Mass Spectrom. 2024, XXXX, XXX, XXX-XXX

https://pubs.acs.org/doi/10.1021/jasms.3c00428

SpheriCal IM-MS: Medium range

SKU: PFS-2002
€350.00Price
    • Type: IM-MS Calibration Standard
    • Delivery Time: starts shipping mid-June 2024
    • m/z Range: 605 - 2,390 
    • CCS Range: 220 - 975 Å2
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