Below you will find protocols for common coupling methodologies that can be used to introduce new biologically active or chemically reactive functionality to the dendritic scaffolds of our dendrimers. Example reactions and representative data are given where appropriate, as well as recommended analytical techniques that can be used to monitor the transformation.
Please note that these protocols are provided for guidance only, and while these chemical modifications are robust and thoroughly investigated by the scientific community, no guarantees are made that they will work for all substrates. Please email us if you have any other queries
Dendritic amine-NHS coupling
Procedure for the coupling of amine-reactive N-hydroxysuccinimide (NHS) activated carboxylic acids to our dendritic scaffolds.
Thiol-ene click functionalization of thiol dendrons
Procedure for the coupling of alkenes to our thiol-functional dendrons via UV-initiated thiol-ene "click."
EDC coupling of amines to dendritic COOH
Procedure for the coupling of amine-containing substrates to our carboxylic acid functional dendritic scaffolds.
(Note: An analogous procedure can be used to functionalize amine function dendritic scaffolds with carboxylic acid-containing substrates)
Deprotection of NHBoc
Procedure for the facile deprotection of NHBoc functional scaffolds, quantitatively revealing primary amine groups for further modification.
CuAAC“Click” coupling of dendritic alkynes/azides
Procedure for the coupling of dendritic alkenes and azides through the efficient and mild "click" chemistry approach.